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Effects of Type II Collagen and Basic‐FGF on Cartilage Wound Healing in a 3D Defect Model
Author(s) -
Chen C.W.,
Tsai Y.H.,
Zuo C.,
Lai W.F.
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.0abstractcv.x
Subject(s) - cartilage , extracellular matrix , chondrocyte , wound healing , regeneration (biology) , type ii collagen , chemistry , matrix (chemical analysis) , type i collagen , microbiology and biotechnology , collagen, type i, alpha 1 , biomedical engineering , anatomy , pathology , immunology , medicine , biochemistry , biology , chromatography
Cartilage preserves limited regenerative capacity and results in insufficient healing after joint injury. Previous researches showed that biocompatible materials and growth factors enable to facilitate cartilage repair; however, the mechanisms of cartilage regeneration are still not completely understood. The purpose of this study was to determine the effects of extracellular matrix and growth factor on chondrocyte migration and phenotype during cartilage regeneration. Neocartilage, 35 mm in diameter and 1 mm in thickness, was fabricated by using rabbit primary chondrocytes embedded in an in vitro three‐dimensional collagen matrix. Five mm diameter defects were made. The effects of collagen matrices and basic‐FGF on three‐dimensional cartilage wound healing were determined. Results showed that both type I and type II collagen matrices (1 mg/ml) facilitated migration of chondrocytes from surrounding cartilage into the defect area in the presence of 10 ng/ml basic‐FGF. The average of migration speed was 91.5micron/day for type I, and 88.1micron/day for type II collagen matrix in the period of 26 days. Incorporation with basic‐FGF, chondrocytes increased approximately 1.5 times motility in the both collagen matrices. However, the cartilaginous phenotype was noted only when embedded in type II collagen matrix. The results indicate that type II collagen may trigger the redifferentiation during the migration of chondrocytes. Extracellular matrix such as type I and type II collagen, in addition to cytokines such as basic‐FGF, play an important role in the process of cartilage wound healing in a three‐dimensional defect model.