Premium
095
HSP70 is Proinflammatory in vivo . Implications for Chronic Wound Healing.
Author(s) -
Perdrizet G,
Enderle J.,
Rewinski M,
Kumar R.,
Enderle G.,
Berman M.,
Hightower L.
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.0abstractco.x
Subject(s) - proinflammatory cytokine , inflammation , medicine , saline , wound healing , in vivo , hsp70 , swelling , debridement (dental) , heat shock protein , downregulation and upregulation , subcutaneous injection , pharmacology , immunology , surgery , pathology , chemistry , biology , biochemistry , microbiology and biotechnology , gene
Failure to downregulate inflammatory responses is often used to explain why chronic wounds fail to heal. The beneficial effects of surgical debridement are thought to be in part due to the transformation of a chronic wound to an acute wound, thereby allowing the inflammatory phase of acute wound healing to be reactivated. We tested the hypothesis that subcutaneous injection of the inducible isoform of the 70 kDa mammalian heat shock protein (iHSP70) will cause acute inflammatory swelling in an in vivo murine model. Comparisons between groups were performed using the Student’s t‐test and significance was accepted when p < 0.05. Subcutaneous injection of stress proteins iHSP70 was associated with significantly increased acute swelling in comparison to injections of saline, autologous serum (AS) or denatured iHSP70, *p < 0.05, see figure below. Histological analysis demonstrated increased inflammatory cell infiltrate in those tissues receiving iHSP70. Stress proteins, within the acute wound environment, are proinflammatory and may set the stage for persistent inflammation within the chronic wound bed. Stress proteins may be future targets for therapeutic manipulation wherever acute inflammation fails to be downregulated.Exp. Group N Swelling(mm)‐24 hrs Swelling(mm)‐ 48 hrs p vs iHSP70Saline 9 0.5 ± 0.25 0.6 ± 0.15 0.001 Serum 8 0.73 ± 0.18 0.72 ± 0.17 0.001 iHSP70 11 1.67 ± 0.36 1.55 ± 0.22 ns Denatured iHSP70 10 1.48 ± 0.41 1.25 ± 0.24 0.05