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The Effect of Localized Intra‐abdominal Infection on Colon Anastomotic Healing in Rats
Author(s) -
Kurt T.,
Tatar A.,
Vladimir F.,
Cassaro S.,
Cioroiu M.,
Grossi R.
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.0abstractbg.x
Subject(s) - hydroxyproline , medicine , anastomosis , wound healing , laparotomy , surgery , body weight , cecum , gastroenterology
Previous studies have demonstrated a decrease in intestinal healing in intra‐abdominal infection. Currently, the effect of localized intra‐abdominal infection on anastomotic healing and strength is not known. This study was designed to evaluate anastomotic wound healing in the presence of intra‐abdominal abscess. 30 male Sprague Dawley rats weighing 270–300 grams were used for this experiment. 20 animals underwent cecal ligation and puncture (CLP) with 16 gauge needle. They were followed for 14 days. 9 of them died in the first week. The CLP animals had an initial 20% weight loss; however by day 10 they had a gradual return toward control weight. At the end of 14 days, rats from CLP group (n = 11) and control group (n = 10) underwent laparotomy and single‐layer left colonic anastomosis. Blood cultures and abdominal swab cultures were sent from both groups. Post‐operatively, animals were allowed to recover, food and water were offered ad libitum . At post‐operative day five, animals were euthanized by thiopental overdose. Following anastomotic bursting pressure measurements, two samples were taken from the anastomotic line for subsequent determination of hydroxyproline content. Groups were compared with Students’t test. Data were expressed as means ± SEM. In the post‐operative period, all rats lost 10% to 12% of their body weight. No bacterial growth was detected in cultures. Hydroxyproline content measurements were significantly higher in the CLP group (11.2 ± 0.3 vs. 9.8 ± 0.5 ug HPO/mg dry tissue weight. P < 0.05). On the other hand, no significant difference was observed in bursting pressure measurements between the groups (177 ± 6.3 vs. 164 ± 10.4 mmHg. P > 0.05). These findings suggest that localized intra‐abdominal infection without sepsis doesn’t inhibit intestinal anastomotic healing. Supported by a grant from Turkish Scientific and Technical Research Council.