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Aged Rats Display Increased Ischemia‐Reperfusion Skin Injury in the in vivo Magnet Model
Author(s) -
Rosenberg David S.,
Lu Leonard,
Gurjala Anandev,
Mustoe Thomas A.
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.0abstractan.x
Subject(s) - medicine , ischemia , in vivo , reperfusion injury , perfusion , anesthesia , surgery , microbiology and biotechnology , biology
: Ischemia‐reperfusion (IR) injury is commonly associated with numerous pathologies including pressure sores, venous stasis ulcers, and lower extremity diabetic ulcers. The purpose of this study is to further investigate the relationship between age and ischemia‐reperfusion skin injury in a rat model utilizing magnets for the purpose of injury creation.
Methods : Magnets were designed for subcutaneous placement and calibrated such that a second magnet placed externally over them would cause compression that exceeds capillary perfusion pressure (ischemia). Removing the external magnet results in reperfusion of the skin. After placing subcutaneous magnets in aged and young Fisher 344 rats, repeated cycles of external magnet placement and removal were performed.
Results : Visual analysis of the skin revealed statistically significant greater areas of injury in the aged rats relative to their younger counterparts (37.4 ± 13.3% vs. 24.1 ± 14.8%, P < .02)
Conclusions : Aged rats demonstrate an increased degree of injury relative to their younger counterparts in response to ischemia‐reperfusion injury. Future studies will attempt to delineate differences in the markers of IR injury (such as myeloperoxidase and vitamin E levels) in aged versus young rats, giving insight to the mechanisms responsible for the impaired wound healing seen in the elderly.