Premium
Structural characteristics of repair tissue of cricoid cartilage defects treated with recombinant human bone morphogenetic protein‐2
Author(s) -
Tcacencu Ion,
Carlsöö Bengt,
Stierna Pontus
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.012307.x
Subject(s) - bone morphogenetic protein , cartilage , bone morphogenetic protein 2 , cricoid cartilage , bone healing , recombinant dna , pathology , anatomy , larynx , medicine , chemistry , biochemistry , gene , in vitro
We examined the structural characteristics of repair tissue induced by recombinant human bone morphogenetic protein‐2 in a rabbit model of laryngotracheal reconstruction. Twenty‐four New Zealand White rabbits were randomly divided into four groups of six rabbits. Two groups were treated with recombinant human bone morphogenetic protein‐2 delivered on an absorbable collagen sponge, while two groups were used as controls. Rabbits were euthanized at 1 and 4 weeks after surgery. The larynx was removed, fixed, and sectioned. The sections were stained with hematoxylin‐eosin, safranine O/fast green, and immunostained with an antibody for tissue inhibitor of metalloproteinases‐1. In rabbits treated with bone morphogenetic protein‐2, the defects were filled with new cartilage and bone at 4 weeks after surgery. There were no discontinuities or gaps at the margins of the cartilage defects. Proteoglycans were synthesized in new cartilage in rabbits treated with bone morphogenetic protein‐2, and were present 4 weeks after surgery. The general aspects of the vascular pattern and the pattern of tissue inhibitor of metalloproteinases‐1 expression were similar in control and treated rabbits, both 1 week and 4 weeks after surgery. The repair tissue induced by recombinant human bone morphogenetic protein‐2 consisted of new cartilage and bone perfectly integrated with host tissue at the site of the cricoid cartilage defects. This new cartilage was able to mature and produce proteoglycans.