Premium
Effects of a CDK inhibitor on lens regeneration
Author(s) -
Tsonis Panagiotis A.,
Madhavan Mayur,
Call Mindy K.,
Gainer Sarah,
Rice Audie,
Del RioTsonis Katia
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.012107.x
Subject(s) - regeneration (biology) , cyclin dependent kinase , lens (geology) , cdk inhibitor , microbiology and biotechnology , chemistry , pharmacology , medicine , biology , biochemistry , cell cycle , cell , paleontology
Lens regeneration in adult newts is always initiated from the dorsal iris by transdifferentiation of the pigment epithelial cells. One of the most important early events should be the ability of pigment epithelial cells to dedifferentiate and re‐enter the cell cycle. As a first step in an attempt to study this event, we have decided to examine the effects of a cyclin‐dependent kinase‐2 inhibitor on lens regeneration. At the appropriate concentration, this inhibitor completely abolished the ability of pigment epithelial cells to form a new lens, but it did not stop them from dedifferentiating and forming a small lens vesicle. The effects of this inhibitor seem to be mediated by its opposite effects on cell proliferation and apoptosis. The inhibitor significantly reduced cell proliferation and enhanced apoptosis of pigment epithelial cells both in vitro and in vivo and of the regenerating lens in vivo.