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Quantitative trait loci affecting dengue midgut infection barriers in an advanced intercross line of Aedes aegypti
Author(s) -
GomezMachorro C.,
Bennett K. E.,
Del Lourdes Munoz M.,
Black W. C.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.0962-1075.2004.00522.x
Subject(s) - biology , quantitative trait locus , aedes aegypti , midgut , genetics , dengue fever , allele , genotype , phenotype , virology , gene , botany , larva
Quantitative trait loci (QTL) affecting the ability of the Aedes aegypti midgut to become infected with Dengue 2 virus (DEN2) were mapped in the F 5 generation of an advanced intercross line (AIL). A strain of Ae. aegypti previously selected for DEN2 susceptibility was crossed to a new strain selected for refractoriness to midgut infection. In P 1 and F 1 parents and 147 F 5 offspring, genotypes at forty‐four cDNA loci were analysed. A new sex linked QTL and a second QTL on chromosome II with genotypes subject to balancing selection were detected that condition midgut susceptibility. Alleles at these QTL contributed additively in determining susceptibility and accounted for ∼24% of the phenotypic variance. Markers associated with a midgut escape barrier were inconsistently supported.