Premium
Baculovirus and dsRNA induce Hemolin, but no antibacterial activity, in Antheraea pernyi
Author(s) -
Hirai M.,
Terenius O.,
Li W.,
Faye I.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.0962-1075.2004.00497.x
Subject(s) - rna silencing , biology , rna interference , antheraea pernyi , gene silencing , lepidoptera genitalia , virology , rna , microbiology and biotechnology , ecology , genetics , gene
Hemolin is one of the haemolymph proteins most strongly induced upon bacterial infection in Lepidoptera. When we applied RNA interference (RNAi) to suppress Hemolin expression in the Chinese oak silk moth Antheraea pernyi , we discovered that Hemolin is induced by double‐stranded RNA (dsRNA) per se. As dsRNA is recognized as a virus pattern molecule, we then investigated the effect of a baculovirus ( Ap NPV) infection. We found that Hemolin is induced and expressed with similar kinetics as upon dsRNA injection. Notably, no Attacin gene expression or antibacterial activity was recorded. When baculovirus and high amounts of dsRNA were coinjected, the viral symptoms appeared earlier with Hemolin dsRNA than with GFP dsRNA. This indicates that silencing of hemolin affected the progress of the viral infection.