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cDNA cloning and transcriptional regulation of the vitellogenin receptor from the imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)
Author(s) -
Chen M.E.,
Lewis D. K.,
Keeley L. L.,
Pietrantonio P. V.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.0962-1075.2004.00477.x
Subject(s) - biology , complementary dna , vitellogenin , juvenile hormone , microbiology and biotechnology , rapid amplification of cdna ends , gene , fire ant , molecular cloning , cloning (programming) , genetics , hymenoptera , botany , insect , computer science , programming language
We describe the cloning of the first hymenopteran vitellogenin receptor (VgR) cDNA from the imported fire ant, Solenopsis invicta , an invasive pest. Using reverse transcription polymerase chain reaction and rapid amplification of cDNA ends, fragments encompassing the entire coding region of a putative VgR were cloned and sequenced. The complete 5764 bp cDNA encodes a 1782 residue protein with a predicted molecular mass of 201.3 kDa (= Si VgR). Northern blot analysis demonstrated that the 7.4 kb Si VgR transcript was present only in ovaries of reproductive females (virgin alates and queens). The temporal profile of transcriptional expression showed that Si VgR mRNA increased with age in virgin alate females and that this was up‐regulated by methoprene, a juvenile hormone (JH) analogue. This suggests that the Si VgR gene is JH regulated.