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Identification and characterization of gp65, a salivary‐gland‐specific molecule expressed in the malaria vector Anopheles albimanus
Author(s) -
MonteroSolis C.,
GonzalezCeron L.,
Rodriguez M. H.,
Cirerol B. E.,
Zamudio F.,
Possanni L. D.,
James A. A.,
De La Cruz HernandezHernandez F.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.0962-1075.2004.00473.x
Subject(s) - anopheles albimanus , biology , complementary dna , microbiology and biotechnology , salivary gland , peptide sequence , amino acid , cdna library , glycosylation , saliva , anopheles , biochemistry , gene , malaria , immunology
A group of salivary‐gland‐specific proteins, designated gp65, were identified in the mosquito Anopheles albimanus . Two‐dimensional gel electrophoresis resolved this group into at least four molecules with pI 6.4–6.5. The N ‐terminal amino acid sequence was determined for the major species, gp65‐1, and degenerate oligonucleotide primers were used to amplify a specific probe for library screening. A 1312 bp cDNA clone encoding a predicted translation product of 386 amino acids was recovered. gp65‐1 is expressed abundantly in the medial and distal–lateral lobes of the adult female glands, and is secreted in the saliva. The amino acid sequence has potential sites for N ‐glycosylation, phosphorylation and myristylation, and is similar to a number of proteins of unknown function from other mosquito species.