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Isolation and characterization of a C‐type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina
Author(s) -
YamamotoKihara M.,
Kotani E.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.0962-1075.2004.00468.x
Subject(s) - arthropod mouthparts , biology , flesh fly , complementary dna , c type lectin , lectin , gene , microbiology and biotechnology , anatomy , larva , genetics , botany
We have isolated a novel gene, CLEM 36 , of the flesh fly Sarcophaga peregrina , which shows significant homology to the C‐type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expressed in the C‐type lectin‐producing tissue (CLPT) located at the entrance of the food canal and between the labellum and haustellum. Immunoblot analysis showed that the mature form of CLEM 36 protein was synthesized in the CLPT, then secreted into saliva. Our results indicate that CLEM 36 protein may play an important role in biological defence against pathogens during the food intake of this insect.