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Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana
Author(s) -
Denby Katherine J.,
Kumar Pavan,
Kliebenstein Daniel J.
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.0960-7412.2004.02059.x
Subject(s) - botrytis cinerea , biology , ecotype , arabidopsis thaliana , quantitative trait locus , arabidopsis , population , epistasis , allele , genetics , botany , gene , mutant , demography , sociology
Summary Botrytis cinerea is a major pathogen of fruit and vegetable crops causing both pre‐ and post‐harvest grey mould. We have analysed 16 Arabidopsis thaliana ecotypes for natural variation in B. cinerea susceptibility. Susceptibility was associated with lower camalexin accumulation, and three ecotypes (Cape Verdi Islands (Cvi‐0), Slavice (Sav‐0) and Kindalville (Kin‐0)) showed differential susceptibility to the two B. cinerea isolates used. Subsequently, to better understand the genetic control of grey mould disease, we assayed the Arabidopsis Landsberg erecta (L er ) × Columbia (Col‐0) recombinant inbred population with the two isolates, and identified multiple small‐to‐medium‐effect quantitative trait loci (QTL) governing susceptibility. Interestingly, the QTL for each isolate are distinct, suggesting that different mechanisms govern defence against these two isolates. Two QTL for each isolate exhibited epistatic interactions with specific allele combinations generating heightened B. cinerea susceptibility.