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Salt stress‐induced chloride flux: a study using transgenic Arabidopsis expressing a fluorescent anion probe
Author(s) -
Lorenzen Inken,
Aberle Thomas,
Plieth Christoph
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.0960-7412.2004.02053.x
Subject(s) - divalent , chloride , chemistry , salt (chemistry) , ion , fluorescence , efflux , biophysics , flux (metallurgy) , ion transporter , osmotic shock , sodium , stress (linguistics) , cytosol , inorganic chemistry , biochemistry , biology , enzyme , organic chemistry , physics , linguistics , philosophy , quantum mechanics , gene
Summary Salt stress leads to massive accumulation of toxic levels of Na + and Cl − ions in plants. By using the recombinant fluorescent probe CLOMELEON, we demonstrate passive anion flux under salt stress. Chloride influx is restricted in the presence of divalent cations like Mg 2+ and Ca 2+ , and completely blocked by La 3+ . The amount but not the rate of the reported chloride uptake is independent from the kind of corresponding permeable cation (K + versus Na + ), external pH and magnitude of osmotic stress. Cl − efflux however seems to involve stretch‐activated transport. From the influence of Ca 2+ on reported changes of cytosolic anion concentrations, we speculate that transport mechanisms of Cl − and Na + might be thermodynamically coupled under saline conditions.