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Evaluation of human knee meniscus biopsies with near‐infrared, reflectance confocal microscopy. A pilot study
Author(s) -
CampoRuiz Vanessa,
Patel Dinesh,
Anderson R. Rox,
DelgadoBaeza Emilio,
González Salvador
Publication year - 2005
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.0959-9673.2005.00439.x
Subject(s) - meniscus , microscopy , materials science , biomedical engineering , cartilage , pathology , confocal microscopy , confocal , medicine , anatomy , optics , physics , incidence (geometry)
Summary Knee cartilage biopsy is used to confirm the pathology in both clinical and experimental conditions and often guides diagnosis and therapeutic strategies. Current histopathological techniques are time consuming, induce tissue artefacts and often prevent further evaluation, once the tissue has been fixed. Hence, there is a potential need for a fast and nondestructive imaging technique for unfixed tissue. Near‐infrared, reflectance confocal microscopy (CM) allows real‐time, virtual sectioning of unstained, bulk tissue samples. This pilot study evaluates the use of CM in the assessment of meniscus histopathology in a series of 26 freshly‐excised human meniscus samples compared to standard light microscopy of stained sections. CM images of the meniscus show cell and matrix detail, depicting morphologic features of collagen and elastic fibres, vessels and nerve endings. In addition, crystal deposits of gout and pseudogout are also demonstrable. Thus, CM is a novel imaging technique that could enable the pathologist to make a rapid microscopic evaluation of cartilage in a fresh and unfixed fashion.