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Radiation sensitivities of 31 human oesophageal squamous cell carcinoma cell lines
Author(s) -
Ban Sadayuki,
Michikawa Yuichi,
Ishikawa Kenichi,
Sagara Masashi,
Watanabe Koji,
Shimada Yutaka,
Inazawa Johji,
Imai Takashi
Publication year - 2005
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.0959-9673.2005.00431.x
Subject(s) - radiosensitivity , ataxia telangiectasia , cancer research , biology , cell culture , cancer , cell , cancer cell , radiation therapy , missense mutation , epidermoid carcinoma , mutation , gene , pathology , genetics , medicine , dna damage , dna
Summary The purpose of this study was to determine the radiosensitivities of 31 human oesophageal squamous cell carcinoma cell lines with a colony‐formation assay. A large variation in radiosensitivity existed among 31 cell lines. Such a large variation may partly explain the poor result of radiotherapy for this cancer. One cell line (KYSE190) demonstrated an unusual radiosensitivity. Ataxia‐telangiectasia‐mutated ( ATM ) gene in these cells had five missense mutations, and ATM protein was truncated or degraded. Inability to phosphorylate Chk2 in the irradiated KYSE190 cells suggests that the ATM protein in these cells had lost its function. The dysfunctional ATM protein may be a main cause of unusual radiosensitivity of KYSE190 cells. Because the donor of these cells was not diagnosed with ataxia telangiectasia, mutations in ATM gene might have occurred during the initiation and progression of cancer. Radiosensitive cancer developed in non‐hereditary diseased patients must be a good target for radiotherapy.