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Current methods for assaying angiogenesis in vitro and in vivo
Author(s) -
Staton Carolyn A.,
Stribbling Stephen M.,
Tazzyman Simon,
Hughes Russell,
Brown Nicola J.,
Lewis Claire E.
Publication year - 2004
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/j.0959-9673.2004.00396.x
Subject(s) - angiogenesis , chorioallantoic membrane , in vivo , in vitro , zebrafish , neovascularization , biology , microbiology and biotechnology , psoriasis , immunology , cancer research , pathology , medicine , biochemistry , gene
Summary Angiogenesis, the development of new blood vessels from an existing vasculature, is essential in normal developmental processes and in numerous pathologies, including diabetic retinopathy, psoriasis and tumour growth and metastases. One of the problems faced by angiogenesis researchers has been the difficulty of finding suitable methods for assessing the effects of regulators of the angiogenic response. The ideal assay would be reliable, technically straightforward, easily quantifiable and, most importantly, physiologically relevant. Here, we review the advantages and limitations of the principal assays in use, including those for the proliferation, migration and differentiation of endothelial cells in vitro , vessel outgrowth from organ cultures and in vivo assays such as sponge implantation, corneal, chamber, zebrafish, chick chorioallantoic membrane (CAM) and tumour angiogenesis models.