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Harvesting peripheral blood progenitor cells from healthy donors with a short course of recombinant human granulocyte‐colony‐stimulating factor
Author(s) -
Martino M.,
Morabito F.,
Callea I.,
Pontari A.,
Irrera G.,
Pucci G.,
Dattola A.,
Messina G.,
Console G.,
Iacopino P.
Publication year - 2005
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.0958-7578.2005.00595.x
Subject(s) - leukapheresis , granulocyte colony stimulating factor , cd34 , medicine , progenitor cell , andrology , immunology , granulocyte , surgery , stem cell , biology , chemotherapy , genetics
summary . A short‐course administration of non‐glycosylated granulocyte‐colony‐stimulating factor (G‐CSF) was investigated in 68 healthy donors (HDs) in order to collect ≥4 × 10 6 CD34 + cells per kilogram of recipient's body weight. G‐CSF was given at 10 µg/kg per day administered in two divided doses for 3 days. Leukapheresis was scheduled on day 4, 12 h after the last dose of G‐CSF. A median of 35·6 circulating CD34 + cells µL −1 (range, 3·1–185) was found on the day of leukapheresis. This allowed a median collection of CD34 + cells of 4·2 × 10 6 per kilogram of recipient's weight (range, 1·0–17·4). One single procedure was sufficient to reach the target level of CD34 + cells in 36 (53%) of 68 donors; significant correlations were found between the number of CD34 + cells collected on day 4 and the patient's sex, body‐weight and volume of blood processed. A retrospective analysis was made with a historical group of HDs collected on day 5. The day 5 schedule allowed a more consistent achievement of the target cell dose with one leukapheresis ( P = 0·005) and resulted in the initial collection of a significantly larger number of CD34 + cells ( P = 0·006).