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Genotyping of human neutrophil antigen‐5a (Ond)
Author(s) -
Sachs U. J. H.,
Reil A.,
Bauer C.,
Bux J.,
Bein G.,
Santoso S.
Publication year - 2005
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.0958-7578.2005.00560.x
Subject(s) - genotyping , polymerase chain reaction , biology , immunology , antibody , typing , antigen , primer (cosmetics) , gene , genotype , genetics , chemistry , organic chemistry
summary . Antibodies to human neutrophil alloantigens (HNA) can cause immune‐mediated neutropenias and transfusion complications. The diagnosis of antibodies to the HNA‐5a (Ond) isoform of the α L β 2 integrin is hampered by the lack of reliable methods for HNA‐5a antigen typing. We have devised a polymerase chain reaction sequence‐specific primer method (PCR‐SSP) and used it to determine the HNA‐5a gene frequencies in 320 individuals from different ethnicities. 15·3% were found to be HNA‐5a negative, with no significant deviation between the populations. Results of HNA‐5a genotyping were in accordance with phenotyping. Availability of HNA‐5a PCR‐SSP will facilitate the diagnosis of Ond antibody‐mediated clinical conditions.