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Interleukin‐1β Stimulates Growth Hormone‐Releasing Hormone Receptor mRNA Expression in the Rat Hypothalamus In Vitro and In Vivo
Author(s) -
Taishi P.,
De A.,
Alt J.,
Gardi J.,
Obal F.,
Krueger J. M.
Publication year - 2004
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.0953-8194.2004.01138.x
Subject(s) - medicine , endocrinology , somatostatin , in vivo , hypothalamus , in vitro , receptor , biology , messenger rna , incubation , hormone , growth hormone–releasing hormone , growth hormone , biochemistry , microbiology and biotechnology , gene
Changes in growth hormone‐releasing hormone (GHRH), GHRH‐receptor (R), somatostatin and interleukin (IL)‐1β mRNA levels were determined in fetal rat hypothalamic cultures after administration of IL‐1β (1, 10, 100 ng/ml, 2 h incubation), and in adult rat hypothalamus 5 h after intracerebroventricular injection of IL‐1β (2.5 and 25 ng). IL‐1β stimulated GHRH‐R mRNA expression both in vitro (10 and 100 ng/ml) and in vivo (2.5 and 25 ng). Somatostatin mRNA was significantly stimulated and GHRH mRNA slightly reduced in vitro , while these mRNA species were not altered in vivo in response to IL‐1β. IL‐1β stimulated its own expression both in vitro (10 and 100 ng/ml) and in vivo (25 ng). IL‐1β‐induced mRNA responses occurred 2 h after treatment in vitro (incubation times, 30 min to 6 h). IL‐1β also elicited slight GHRH releases in vitro . Up‐regulation of hypothalamic GHRH‐R by IL‐1β may explain previous findings suggesting that IL‐1β stimulates GHRH activity.