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Recruitment of osteoclasts in the mandible of osteopetrotic ( op/op ) mice
Author(s) -
IdaYonemochi Hiroko,
Ishibashi Osamu,
Sakai Hideaki,
Saku Takashi
Publication year - 2004
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.0909-8836.2004.00109.x
Subject(s) - acid phosphatase , periodontal fiber , mandible (arthropod mouthpart) , cathepsin k , osteoclast , connective tissue , anatomy , medicine , chemistry , endocrinology , biology , pathology , dentistry , biochemistry , botany , receptor , genus , enzyme
Osteoclasts in osteopetrotic ( op/op ) mice are substantially reduced by the absence of functional activities of macrophage colony‐stimulating factor (M‐CSF). However, it is known that osteoclasts appear in op/op skeletal bones with aging, although the molecular mechanism for this is unknown. In order to investigate osteoclastic recruitment in the jaw bones of op/op mice, osteoclastic distribution was analysed for 2 yr after birth by histochemistry for tartrate‐resistant acid phosphatase activity and immunohistochemistry for cathepsin K. Osteoclasts in op/op mandibular bones decreased rapidly in number after birth and disappeared by 3 d, although there was no difference in the osteoclastic distribution between op/op and normal littermates at birth. At 2 wk, osteoclasts began to reappear around op/op tooth germs, where no apparent connective tissue layer intervened between tooth germs and bone trabeculae. They increased in number and were scattered over the mandible, reaching a maximum at 8 wk, when periodontal ligament‐like structures were recognizable around incisor germs. Osteoclasts then again decreased gradually, and after 62 wk few osteoclasts were seen in op/op mandibular bones, whose marrow space disappeared. These findings suggest that osteoclasts are recruited in an M‐CSF‐independent manner in op/op mandibles, especially in areas around tooth germs.