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T‐cell costimulatory capacity of oral and skin epithelial cells in vitro: presence of suppressive activity in supernatants from skin epithelial cell cultures
Author(s) -
Hasséus B.,
Jontell M.,
Bergenholtz G.,
Dahlgren U. I.
Publication year - 2004
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.0909-8836.2004.00097.x
Subject(s) - biology , flow cytometry , t cell , microbiology and biotechnology , immunology , immune system
Oral Langerhans cells (LC) have better T‐cell costimulatory capacity than skin LC. In this study factors affecting this capacity have been assessed in a mixed epithelial cell lymphocyte reaction (MELR) assay. Flow cytometry analysis of freshly recovered cells revealed major histocompatibility complex (MHC) class II molecule expression on 7.5% of the oral epithelial cells and 9.7% of the skin epithelial cells. Monoclonal anti class II antibodies significantly reduced the T‐cell proliferation in the MELR. Pretreatment of skin epithelial cells with interleukin‐1β, tumour necrosis factor‐α or interferon (IFN)‐γ did not affect the MELR proliferation, but incubation with IFNγ significantly suppressed the T‐cell response. Transfer of supernatants from cultures of skin epithelial cells and allogeneic T cells to cultures of oral epithelial cells and T cells resulted in a reduced T‐cell proliferation while supernatants from oral epithelial cells and T cells did not reduce proliferation. The higher proliferation in cultures of T cells and oral epithelial cells than in cultures containing skin epithelial cells may be due to the presence of a suppressive factor in the skin epithelial cell suspensions.