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Involvement of urokinase‐type plasminogen activator in sphingosylphosphorylcholine‐induced angiogenesis
Author(s) -
Piao YongJun,
Lee ChangHi,
Zhu Ming Ji,
Kye KyungChae,
Kim JinMan,
Seo YoungJoon,
Suhr KiBeom,
Park JangKyu,
Kim Chang Deok,
Lee JeungHoon
Publication year - 2005
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.0906-6705.2005.00272.x
Subject(s) - angiogenesis , wound healing , umbilical vein , plasminogen activator , chemistry , chemotaxis , microbiology and biotechnology , endothelial stem cell , neovascularization , human umbilical vein endothelial cell , urokinase , in vitro , cancer research , immunology , biology , endocrinology , biochemistry , medicine , receptor
Abstract: Sphingosylphosphorylcholine (SPC) has been shown to accelerate wound healing. As angiogenesis is fundamental to proper wound healing, we examined the effect of SPC on angiogenesis using a well‐established rat aortic ring assay. SPC significantly stimulated the sprouting of endothelial cells from rat aortic ring. Recognizing its potential effect on angiogenesis, we further investigated the action of SPC using human umbilical vein endothelial cells (HUVECs) cultured in vitro . SPC significantly accelerated the closure of in vitro wound. In addition, SPC markedly enhanced the chemotactic migration and capillary‐like tube formation. Subsequently, we examined whether SPC affected the production of urokinase‐type plasminogen activator (uPA), an important regulator of angiogenesis, and found that SPC stimulated the expression of uPA at both the transcriptional and translational levels. Consistent with these results, SPC increased the activity of cell‐surface‐associated plasminogen activator. Pretreatment with antiuPA antibody significantly diminished both the chemotactic migration and capillary‐like tube formation, indicating the potential importance of uPA in SPC‐induced angiogenesis. Together, these results suggest that SPC may affect angiogenesis in the wound‐healing process via regulation of uPA production.