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Defective hair formation in calcitonin‐like receptor transgenic mice
Author(s) -
Ittner L. M.,
Husmann K.,
Muff R.,
Götz J.,
Bonneick S.,
Suter U.,
Born W.,
Fischer J. A.
Publication year - 2004
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.0906-6705.2004.212bg.x
Subject(s) - genetically modified mouse , transgene , hair cycle , receptor , in situ hybridization , adrenomedullin , endocrinology , medicine , biology , calcitonin , calcitonin receptor , hair follicle , microbiology and biotechnology , chemistry , calcitonin gene related peptide , messenger rna , neuropeptide , biochemistry , gene
The heterodimeric calcitonin‐like receptor (CLR)/receptor‐activity‐modifying protein 2 (RAMP2) complex is an adrenomedullin (AM) receptor. Here, transgenic mice expressing a V5‐CLR in hair follicles have been generated. Skin sections of the transgenic mice and of control littermates have been investigated. The hair diameter of the mice was measured during hair morphogenesis and first hair cycle. Size and weight of V5‐CLR transgenic and control mice were indistinguishable. But after day 12, the coat of the transgenic mice is waved. The number of hairs with a small diameter was significantly higher in transgenic mice as compared with control littermates ( P < 0.001). There skin sections revealed immunoreactive V5‐CLR in the bulb of cycling hair follicles. On in situ hybridization, RAMP2 encoding mRNA colocalized with the V5‐CLR protein. Autoradiographic examination showed specific 125 I‐AM binding in the same place. In conclusion, CLR/RAMP2‐overexpressing mice reveal a defined phenotype with thinning of the hairs during postnatal development.