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Neurotrophins exert immunomodulatory functions on peripheral blood eosinophils in atopic dermatitis
Author(s) -
Raap U.,
Deneka N.,
Goltz C.,
Bruder M.,
Renz H.,
Kapp A.,
Wedi B.
Publication year - 2004
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.0906-6705.2004.0212cg.x
Subject(s) - respiratory burst , eosinophil , nerve growth factor , neurotrophin , stimulation , immunology , chemotaxis , neurotrophic factors , brain derived neurotrophic factor , medicine , endocrinology , asthma , receptor
Recently, the functional role of neurotrophins on eosinophils in allergic asthma has been described. The aim of this study was to investigate the possible role of BDNF, NT‐3, NT‐4 and NGF on peripheral blood eosinophils in atopic dermatitis (AD). AD patients were defined according to the criteria of Hanifin and Rajika. Peripheral blood eosinophils were purified by CD16‐negative selection (purity >98%) and stimulated with BDNF, NT‐3, NT‐4 (10, 50 and 100 ng/ml), or NGF (100, 500 and 1000 ng/ml) for 24 up to 120 h. Apoptotic eosinophils were investigated by determining their hypodiploid DNA peak and Annexin V method, respectively. Chemotactic index was assessed in a modified Boyden chamber assay and respiratory burst by lucigenin‐dependent chemiluminescence. Stimulation with BDNF, NT‐3 and NGF significantly inhibited the programmed cell death of AD eosinophils ( P < 0.05–0.01) at each time point (24 h up to 120 h in culture). Chemotactic index was significantly increased in AD eosinophils after stimulation with BDNF, NT‐3 and NT‐4 ( P < 0.05–0.01). Respiratory burst of AD eosinophils was not modified after stimulation with BDNF, NT‐3, NT‐4 or NGF. To summarize, BDNF, NT‐3 and NGF significantly inhibited the programmed cell death of AD eosinophils. However, BDNF, NT‐3, NT‐4 and NGF did not modulate respiratory burst of AD peripheral blood eosinophils. On the other hand, BDNF, NT3 and NT‐4 significantly induced AD eosinophil chemotaxis. Taken together, neurotrophins have a functional role on peripheral blood eosinophils revealing neuro–immunological interactions in AD. Supported by HILF grant.