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Increased intraepidermal CGRP correlates with local immuno‐suppression after repeated broadband and narrowband UVB
Author(s) -
Legat F. J.,
Wolf P.,
Jaiani L. T.,
Lang R.,
Wang M. S.,
Armstrong C. A.,
Ansel J. C.,
Glass J. D.
Publication year - 2004
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.0906-6705.2004.0212bs.x
Subject(s) - hairless , sensitization , calcitonin gene related peptide , medicine , erythema , phototoxicity , pharmacology , chemistry , immunology , in vitro , receptor , neuropeptide , biochemistry
We investigated the effects of repeated broadband UVB (UVB‐BB) and repeated narrowband UVB (UVB‐NB) on intraepidermal nerve fibers (ENF) immunoreactive (IR) for CGRP and on CHS to DNFB in hairless mice. Mice were exposed to equivalent subinflammatory UVB‐BB (Kodacel‐filtered FS20) or UVB‐NB (TL01) 3× per week for 4 weeks. One, 3, or 7 days after the last UV exposure, the number of CGRP‐IR ENF was determined in exposed back skin. At the same time points, other mice were sensitized to DNFB on exposed back skin, challenged on abdominal skin 5 days later, and skin swelling was determined at 24 h after challenge. UVB‐BB and UVB‐NB significantly increased the number of CGRP‐IR ENF in exposed back skin and significantly suppressed the sensitization of animals to DNFB. For both treatments, these effects were maximal 1 day after the last UV exposure. However, at 7 days after the last UV exposure, in UVB‐NB‐irradiated animals, the number of CGRP‐IR ENF was still significantly increased and the sensitization to DNFB significantly suppressed, while in UVB‐BB‐irradiated animals, these parameters were similar to that in non‐irradiated controls. Thus, while repeated exposure to UVB‐BB or UVB‐NB or both induced increase of CGRP‐IR ENF as well as local immunosuppression, the effects after repeated UVB‐NB were prolonged compared with that after repeated UVB‐BB. This may contribute to the higher phototherapeutic efficacy of UVB‐NB vs. UVB‐BB.