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N ‐methyl‐ D ‐aspartate receptors influence the intracellular calcium concentration of keratinocytes
Author(s) -
Fischer Matthias,
Glanz Dagobert,
William Thomas,
Klapperstück Thomas,
Wohlrab Johannes,
Marsch Wolfgang CH.
Publication year - 2004
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.0906-6705.2004.00200.x
Subject(s) - nmda receptor , hacat , stratum granulosum , ionotropic effect , chemistry , receptor , calcium in biology , microbiology and biotechnology , calcium , glutamate receptor , biology , biochemistry , stratum corneum , in vitro , organic chemistry , genetics
In the present study, the distribution of ionotropic glutamate receptors of the N ‐methyl‐ D ‐aspartate (NMDA)‐receptor type was immunohistochemically demonstrated in healthy human skin ( n = 22) and healthy buccal mucosa ( n = 20). Moreover, the intracellular calcium concentration of HaCaT‐cells and native human keratinocytes were studied under the influence of the selective agonist NMDA and the selective NMDA‐antagonist MK‐801. Immunohistochemical imaging of NMDA receptors in healthy epidermis showed a positive reaction in the stratum basale, spinosum and granulosum, whereby the greatest expression was observed in the granular layer. In the mucosal preparations, the distribution of NMDA receptors was observed to be equal in all cell layers. In the cell culture (HaCaT‐cells), NMDA concentrations between 25 µM and 1 mM resulted in a significant increase in the number of cells showing elevated intracellular calcium concentration. This effect could be significantly reduced by prior application of MK‐801 (100 µM). In supplementary tests on HaCaT‐keratinocytes, blockade of the keratinocytic NMDA receptors with MK‐801 suppressed the differentiation of the cells (expression of cytokeratin 10). The proliferation of cells was not influenced by NMDA. The investigations showed that glutamate receptors of the NMDA type have an influence on keratinocytic calcium concentration. This appears especially important for the differentiation of keratinocytes.