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Contact sensitizer nickel sulfate activates the transcription factors NF‐kB and AP‐1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
Author(s) -
Cruz M. Teresa,
Gonçalo Margarida,
Figueiredo Américo,
Carvalho Arsélio P.,
Duarte Carlos B.,
Lopes M. Celeste
Publication year - 2004
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.0906-6705.2004.00105.x
Subject(s) - nitric oxide synthase , transcription factor , nitric oxide , microbiology and biotechnology , electrophoretic mobility shift assay , chemistry , western blot , transcription (linguistics) , nfkb1 , biochemistry , biology , gene , organic chemistry , linguistics , philosophy
Nuclear factor kappa B (NF‐kB) and activating protein‐1 (AP‐1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin‐derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO 4 ) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO 4 increased cytoplasmic iNOS expression by 31.9 ± 10.3% and nitrite production, as assayed by the Griess reaction, by 27.6 ± 9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30 min of FSDC exposure to NiSO 4 activates the transcription factor NF‐kB by 58.2 ± 7.0% and 2 h of FSDC exposure to NiSO 4 activates the transcription factor AP‐1 by 26.0 ± 1.4%. Together, these results indicate that NiSO 4 activates the NF‐kB and AP‐1 pathways and induces iNOS expression in skin dendritic cells.