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The Th1/Th2 immune‐type response of the recurrent aphthous ulceration analyzed by cDNA microarray
Author(s) -
Borra R. C.,
Andrade P. M.,
Silva I. D. C. G.,
Morgun A.,
Weckx L. L. M.,
Smirnova A. S.,
Franco M.
Publication year - 2004
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.0904-2512.2004.00089.x
Subject(s) - microarray , complementary dna , immune system , pathogenesis , microarray analysis techniques , gene expression , biology , immunology , gene , dna microarray , gene chip analysis , recurrent aphthous stomatitis , medicine , genetics , stomatitis
Background: The reduced ability to activate oral tolerance plays a role in the pathogenesis of some gastrointestinal inflammatory diseases. This activation may reflect a preferential reduction of a T‐helper (Th)2‐ or Th3‐type response. In recurrent aphthous ulceration (RAU), genetic and environmental factors may contribute to low tolerance, permitting a cytotoxic reaction against the oral epithelium. The cytokine profile has not permitted the definition of RAU as resulting from enhanced Th1 or Th2 responses. A cDNA microarray study would allow the identification of differentially expressed genes and provide a basis for classification of the immune response. Methods: The cDNA from 29 samples of aphthae and from 11 samples of normal mucosa from aphthae‐free volunteers were hybridized on microarray membranes with 1176 genes. Results: Forty‐one differentially expressed genes were identified, and a higher expression level of the Th1 gene cluster in RAU was found. Conclusions: Microarrays permitted us definition of the gene expression profile of the lesion and identify an increased Th1 activity in RAU lesions.