Premium
Liposome‐stabilized oil‐in‐water emulsions as adjuvants: Increased emulsion stability promotes induction of cytotoxic T lymphocytes against an HIV envelope antigen
Author(s) -
Richards Roberta L,
Rao Mangala,
Vancott Thomas C,
Matyas Gary R,
Birx Deborah L,
Alving Carl R
Publication year - 2004
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/j.0818-9641.2004.01282.x
Subject(s) - adjuvant , liposome , ctl* , antigen , emulsion , antibody , chemistry , cytotoxic t cell , immune system , biology , immunology , biochemistry , in vitro , cd8
Protective or therapeutic immunity against HIV infection is currently believed to require both antibody and CTL responses against the envelope protein. In the present study, the adjuvant activity of a unique oil‐in‐water emulsion, in which liposomes containing lipid A (LA) and encapsulated antigen served as the emulsifying agent, was examined in mice using oligomeric gp140 (ogp140) derived from the HIV‐1 envelope as the antigen. Emulsions rendered either highly stable or unstable by altering the ratio of liposomes to oil were used to examine the effect of stability of the emulsion on adjuvant activity. Stable and unstable emulsions had similar potencies for inducing both IgG antibodies to ogp140 and antigen‐specific T‐lymphocyte proliferation. Stable emulsions, but not unstable emulsions, induced antigen‐specific CTL responses, possibly because of the depot effect of the stable emulsions. Furthermore, stable emulsions induced lower IgG2a/IgG1 ratios than the unstable emulsions. We conclude that stable liposomal oil‐in‐water emulsions provide an effective means of obtaining both antibody and CTL responses against an HIV envelope antigen.