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GRAFT‐VERSUS‐HOST ACTIVITY OF RAT LYMPHOCYTES AFTER SENSITIZATION IN VITRO
Author(s) -
Nielsen Hans Erik
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.0365-5563.1973.tb00013.x
Subject(s) - in vitro , immune system , antigen , sensitization , histocompatibility , immunology , in vivo , biology , antibody , immunization , microbiology and biotechnology , human leukocyte antigen , genetics
In order to test the hypothesis that the small increase in graft‐versus‐host (GVH) activity after immunization in vivo against strong histocompatibility antigens may be due to an inhibitory effect of allo‐antibody on the cellular immune response, rat lymphocytes were sensitized in vitro with allogeneic lymphocytes. Peripheral lymphocytes from inbred Fischer (F) strain rats were cultured in vitro either alone or together with (F × BN) F1 lymphocytes in conditions where humoral antibodies are not demonstrably formed. After 4 and 6 days of culture the activity of the stimulated and non‐stimulated cells was compared in a GVH assay using (F × BN) F1 and (F × AS2) F1 hybrids as hosts. The activity of the in vitro sensitized cells against (F × BN) F1 recipients after four and six days of culture was not significantly enhanced. These findings support the alternative hypothesis that a very high proportion of cells are in fact sensitive to histocompatibility antigens, even in the non‐immune animal.