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SOME CHARACTERISTICS OF THE A ANTIGEN IN MAREK'S DISEASE VIRUS—INFECTED CELL CULTURES
Author(s) -
Settnes O. P.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.0365-5563.1973.tb00006.x
Subject(s) - antigen , virology , marek's disease , trypsin , virus , centrifugation , inoculation , cell culture , biology , interferon , cell , microbiology and biotechnology , immunology , enzyme , biochemistry , genetics
The A antigen in Marek's Disease Virus infected cell cultures was found to be stable at 37° C or lower for long periods, to be rapidly inactivated at temperatures at 75° C to 100° C, unaffected by ether or chloroform, tolerate pH 1.7 for 4 1/2 hours, but is inactivated by pH 12.1 in 4 1/2 hours. Trypsin inactivates the antigen activity in 40–60 minutes at 37° C and it remains in the supernatant after centrifugation at 100,000 × G for 120 minutes. A antigen preparations were found inhibitory to the replication of MDV in cell cultures and to VSV plaque formation in CK cultures, but not in L cells. The possibility that the A antigen is an interferon ‐associated protein is discussed.