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Immunohistochemistry of the B‐Cell Component in Lower Lip Salivary Glands of Sjögren's Syndrome and Healthy Subjects
Author(s) -
Larsson Å.,
Bredberg A.,
Henriksson G.,
Manthorpe R.,
Sallmyr A.
Publication year - 2005
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.0300-9475.2005.01540.x
Subject(s) - immunohistochemistry , salivary gland , pathology , medicine , lower lip , sjögren syndrome , dermatology , disease , autoimmune disease , surgery
Serial sections of lower lip salivary gland (LSG) biopsies were examined by immunohistochemistry, using a battery of B‐ and partly T‐related antibodies (CD5, CD20, CD21, CD27, CD38, CD45RO, CD79a, Bcl‐2 and Bcl‐6) in different groups of subjects: healthy controls and clinically verified smoking or nonsmoking cases of primary Sjögren's syndrome (SS). The purpose was to characterize the B‐cell pattern of the lymphocytic foci and of the tiny perivascular infiltrates preceding the development of foci. Hyperplastic tonsil was used as stain control. In normal LSG, widely dispersed CD38 + and CD79a + as well as some CD5 + cells are a normal constituent, with lack of staining with the other antibodies. In SS/LSG, the lymphocytic foci showed staining with all the antibodies, with variable degrees of overlapping or nonoverlapping. In SS/LSG of nonsmokers, CD20 + B cells make up a prominent part of the fully developed periductal lymphocytic foci, not overlapping with CD45RO. Also, CD20 + B cells did not overlap in the infiltrates with colocalized CD27 + /CD38 + cells. CD20 + B cells and CD45RO + T cells also occur as minute infiltrates perivascularly in areas of no foci in SS/LSG as well as in SS smokers lacking the typical foci. Smokers lack foci, but tiny infiltrates express CD20 as well CD45R0. Our findings suggest that CD20 + B cells and CD45RO + T cells are early immigrants in the LSG of SS of smokers as well as nonsmokers and that another subgroup of CD27 + /CD38 + B cells gradually mix with the first two to form the characteristic foci in SS/LSG. The simultaneous demonstration of CD20 + and CD27 + B cells in SS/LSG may constitute a significant diagnostic tool. Further, the findings suggest that the early immigrating lymphocytes may have been primed at a site remote from the glands before arriving via the blood to the gland tissue.