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Culture of Regulatory T‐Cell Lines from Bronchial Mucosa
Author(s) -
Rømer J. L.,
Kelsen J.,
Dahl R.,
Hoffmann H. J.
Publication year - 2004
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.0300-9475.2004.01423z.x
Subject(s) - sarcoidosis , il 2 receptor , immunology , immune system , effector , phenotype , homeostasis , biology , function (biology) , t cell , medicine , pathology , microbiology and biotechnology , gene , genetics
T lymphocytes play a major role in many immune responses. In the last decade, special focus has been on the function of Th1 and Th2 effector cells. Now the importance of regulatory CD4 + CD25 + T cells in maintenance of the immunological homeostasis emerges. Sarcoidosis is a multisystem granulomatous disorder often affecting the lungs. The typical sarcoid granulomas consists of epitheloid cells, macrophages and lymphocytes, mainly CD4 + T cells of Th1 phenotype. We have cultured T cells from bronchial biopsies of patients with sarcoidosis as well as from controls in high levels of interleukin 2 (IL‐2) and IL‐4 and demonstrate spontaneously arising CD4 + CD25 + populations and high concentrations of IL‐10 in these cultures. The main difference between cultures of sarcoid origin compared to controls is a very much higher concentration of the inflammatory cytokines IL‐6 and TNF‐α in cultures of sarcoid origin.