Premium
Induction of a Mucosal Immune Response to the Streptococcal M Protein by Intramuscular Administration of a PADRE‐ASREAK Peptide
Author(s) -
Pamonsinlapatham P.,
Decroix N.,
MihailaAmrouche L.,
Bouvet A.,
Bouvet J.P.
Publication year - 2004
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.0300-9475.2004.01421.x
Subject(s) - antibody , immune system , epitope , antigen , peptide , biology , immunoglobulin g , immunofluorescence , microbiology and biotechnology , immunology , immunoglobulin a , intramuscular injection , virology , medicine , biochemistry
In a previous study, it was shown that an intramuscular administration of amino acid PADRE‐ ELDKWA sequence induced a mucosal immune response to a conserved epitope of human immunodeficiency virus in mice. In the same model, here it is shown that this method can be used with a selected peptide from the M protein of group A streptococci. The PADRE‐ ASREAK sequence was injected in mice by the intramuscular route. Antibodies against M protein were detected in extracts of mucosal tissues and in serum. The repertoire isotypes of serum immunoglobulin G (IgG) and mucosal IgA and IgG antibodies varied, according to the dose of injected peptide. The highest mucosal IgA antibody response was obtained with 0.01 µg of antigen per injection, whereas the systemic IgG antibody response increased with 10 µg of antigen. Mucosal antibody production against streptococci was confirmed by immunofluorescence analysis. These results provide evidence that this novel approach of mucosal vaccination may be of advantage for bacterial systems and suggest a new field of investigation based on synthetic peptide analogues.