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Interleukin‐4 Selectively Inhibits Interleukin‐2 Secretion by Lipopolysaccharide‐Activated Dendritic Cells
Author(s) -
Sauma D.,
Michea P.,
LenDuménil A.M.,
Fierro A.,
Morales J.,
Rosemblatt M.,
Bono M. R.
Publication year - 2004
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.0300-9475.2004.01380.x
Subject(s) - secretion , dendritic cell , immune system , lipopolysaccharide , priming (agriculture) , microbiology and biotechnology , interleukin 12 , cytokine , interleukin , chemistry , immunology , biology , in vitro , cytotoxic t cell , endocrinology , biochemistry , botany , germination
Dendritic cells (DCs) generated in vitro from bone marrow precursors using granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) secrete interleukin‐2 (IL‐2) upon activation, an event probably associated to the initiation of adaptive immune responses. Additionally, they produce IL‐12, a cytokine related to T‐cell polarization. To analyse the effect of IL‐4 on DC differentiation and function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) differentiated with GM‐CSF in the presence or absence of IL‐4 to produce IL‐2 and IL‐12 upon lipopolysaccharide (LPS) activation. We found that although IL‐4 enhanced DC IL‐12p70 production, it strongly impaired IL‐2 secretion by BMDCs. This inhibition, which depends on the presence of IL‐4 during LPS activation, is DC specific, as IL‐4 did not affect IL‐2 secretion by T cells. Interestingly, inhibition of DC IL‐2 production did not prevent DC priming of T lymphocytes. These results illustrate a new putative role for IL‐4 on the regulation of the immune response and should help clarify the controversial reports on the effect of IL‐4 on DCs.