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Ligation of CD47 During Monocyte Differentiation into Dendritic Cells Results in Reduced Capacity for Interleukin‐12 Production
Author(s) -
Johansson U.,
Londei M.
Publication year - 2004
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.0300-9475.2004.01354.x
Subject(s) - cd47 , microbiology and biotechnology , tumor necrosis factor alpha , monocyte , apoptosis , interleukin , follicular dendritic cells , interleukin 10 , lipopolysaccharide , dendritic cell , biology , chemistry , immune system , immunology , t cell , cytokine , phagocytosis , antigen presenting cell , biochemistry
We have previously shown that the CD47‐binding thrombospondin‐1 (TSP‐1)‐derived peptide 4N1K induces a rapid apoptosis‐like death of human monocytes and dendritic cells (DCs). However, not all cells were susceptible to the peptide‐induced cell death and here, we have investigated whether surviving monocytes could differentiate into functionally normal DCs. We found that the cell‐surface phenotype, the T‐cell stimulatory capacity and the ability to undergo lipopolysaccharide (LPS)‐induced maturation into CD83 + DCs were essentially identical in 4N1K‐derived and control DCs. Interleukin‐10 (IL‐10) production was also normal, but a significant downregulation of the pro‐inflammatory cytokines IL‐12 and tumour necrosis factor‐α (TNF‐α) was observed in the 4N1K‐derived DCs. To the contrary, simultaneous stimulation of control DCs with 4N1K and LPS + interferon‐γ did not alter IL‐12 production. These results indicate that although activation of the TSP‐1‐binding region of CD47 on monocytes induces apoptosis in a large proportion of the cells, it does not hamper the overall capacity of the surviving cells to differentiate into DCs. Such DCs, however, have a reduced capacity for IL‐12 and TNF‐α production, and the possibility that this is linked to the uptake of apoptotic cells is discussed.