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Down regulation of Hsp70 expression level prolongs the duration of heat‐induced male sterility in Drosophila buzzatii
Author(s) -
SARUP P.,
DAHLGAARD J.,
NORUP A.M.,
JØRGENSEN K. T.,
HEBSGAARD M. B.,
LOESCHCKE V.
Publication year - 2004
Publication title -
functional ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 154
eISSN - 1365-2435
pISSN - 0269-8463
DOI - 10.1111/j.0269-8463.2004.00863.x
Subject(s) - sterility , biology , hsp70 , andrology , pupa , drosophila melanogaster , heat shock protein , toxicology , genetics , larva , botany , gene , medicine
Summary1 The relationship between heat shock protein Hsp70 expression level and the duration of heat‐induced male sterility was investigated in four populations of Drosophila buzzatii Patterson and Wheeler. The effect of heat hardening on the duration of sterility was further examined after flies developed at either 25 or 31 °C. In addition, Hsp70 expression was measured in testes after development at three different thermal regimes. 2 Four main hypotheses were tested: (i) Hsp70 is expressed in testes of D. buzzatii males even at non‐stressful temperatures, and the level of expression increases with increasing rearing temperature. (ii) Hsp70 expression level differs between populations and is negatively correlated with the duration of poststress sterility. (iii) Experimentally induced Hsp70 expression at the pupal stage shortens the sterility period of flies reared above the temperature threshold of sterility. (iv) In contrast, a hardening treatment during the pupal stage of flies reared at 25 °C results in a longer time to fertility. 3 The results matched the hypotheses, leading to the conclusion that higher Hsp70 expression reduces the duration of heat‐induced male sterility.