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Role of substance P and calcitonin gene‐related peptide in the regulation of interleukin‐8 and monocyte chemotactic protein‐1 expression in human dental pulp
Author(s) -
Park S. H.,
Hsiao G. Y.W.,
Huang G. T.J.
Publication year - 2004
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.0143-2885.2004.00782.x
Subject(s) - calcitonin gene related peptide , pulp (tooth) , chemokine , monocyte , substance p , neurogenic inflammation , calcitonin , chemistry , interleukin 8 , inflammation , pulpitis , proinflammatory cytokine , microbiology and biotechnology , immunology , endocrinology , biology , neuropeptide , medicine , biochemistry , dentistry , receptor
Aim To determine whether leucocyte infiltration during neurogenic inflammation in the pulp is regulated by neuropeptides via inducing the release of proinflammatory chemokines interleukin‐8 (IL‐8) and monocyte chemotactic protein‐1 (MCP‐1) from human dental pulp. Methodology Cultured primary pulp cells and pulp tissue explants were stimulated with substance P (SP) and/or calcitonin gene‐related peptide (CGRP). IL‐8 or MCP‐1, secreted from cultured cells or produced in pulp explants, was analysed by enzyme‐linked immunosorbent assay. Results Substance P induced IL‐8 secretion from cultured pulp cells (approximately threefold increase over control, P < 0.05) and from pulp tissue explants (two‐ to three fold). SP only minimally to moderately induced MCP‐1 (approximately two fold) in cultured pulp cells. While MCP‐1 induction in cultured pulp cells was detected after 24 h of SP stimulation, no induction was observed in pulp tissue. CGRP did not induce IL‐8, but moderately increased MCP‐1 production (approximately three fold) in cultured pulp cells. There was no synergistic induction of MCP‐1 by SP plus CGRP stimulation of pulp cells. Conclusions Substance P is a stronger inducer of IL‐8 production in dental pulp than CGRP. IL‐8 is more strongly induced than MCP‐1 by SP, suggesting a more important role for IL‐8 than MCP‐1 in leucocyte infiltration during neurogenic inflammation in dental pulp.