Premium
Trichomonas vaginalis ‐induced apoptosis in RAW264·7 cells is regulated through Bcl‐x L , but not Bcl‐2
Author(s) -
Chang J.H.,
Ryang Y.S.,
Kim S.K.,
Park J.Y.
Publication year - 2004
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.0141-9838.2004.00693.x
Subject(s) - apoptosis , trichomonas vaginalis , cytochrome c , biology , poly adp ribose polymerase , programmed cell death , caspase , transfection , caspase 3 , microbiology and biotechnology , uvb induced apoptosis , bcl 2 associated x protein , cancer research , cell culture , gene , genetics , polymerase
SUMMARY The purpose of this study was to determine whether anti‐apoptotic proteins of the Bcl‐2 family such as Bcl‐2 and Bcl‐xL, proteins that confer resistance to apoptotic death from some stimuli, block apoptotic cell death in RAW264·7 cells upon treatment with Trichomonas vaginalis. In this study, the expression level of Bcl‐2 was unchanged throughout the course of apoptotic cell death, and overexpressed Bcl‐2 did not prevent release of cytochrome c, the significant change of the membrane potential, activation of caspases, and PARP cleavage in T. vaginalis ‐treated RAW264·7 cells. On the other hand, Bcl‐x L expression was decreased after T. vaginalis treatment accompanied with Bax activation. Furthermore, we showed that release of mitochondrial cytochrome c, cleavage of caspase‐9 and PARP during apoptosis in T. vaginalis ‐treated RAW264·7 cells were considerably diminished by transfection with overexpressed Bcl‐x L , and overexpressed Bcl‐x L could inhibit T. vaginalis ‐induced apoptosis in RAW264·7 cells. In addition, interestingly, pre‐treatment with caspase inhibitors, Boc‐D‐FMK and Z‐DEVD‐FMK, significantly abolished T. vaginalis ‐induced down‐regulation of Bcl‐x L , suggesting that caspase‐3 may play a pivotal role in the process of apoptosis as well as the down‐regulation of Bcl‐x L by T. vaginalis . Therefore, these results suggest that T. vaginalis ‐induced apoptosis in RAW264·7 cells can occur via a Bcl‐x L ‐dependent apoptotic mechanism.