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Use of baculovirus MHC/peptide display libraries to characterize T‐cell receptor ligands
Author(s) -
Crawford Frances,
Jordan Kimberly R.,
Stadinski Brian,
Wang Yibing,
Huseby Eric,
Marrack Philippa,
Slansky Jill E.,
Kappler John W.
Publication year - 2006
Publication title -
immunological reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.839
H-Index - 223
eISSN - 1600-065X
pISSN - 0105-2896
DOI - 10.1111/j.0105-2896.2006.00365.x
Subject(s) - biology , phage display , mimotope , major histocompatibility complex , peptide library , antigen presentation , antigen , computational biology , mhc class i , peptide , microbiology and biotechnology , t cell , peptide sequence , genetics , immune system , biochemistry , gene
Summary: Peptide/protein display libraries are powerful tools for identifying and manipulating receptor/ligand pairs. While the large size of bacterial phage display libraries has made them the platform of choice in many applications, often considerable engineering has been required to achieve display of properly folded and active eukaryotic proteins, such as antibodies. This problem has been partially solved in several eukaryotic display systems, e.g. using yeast or retroviruses, but these systems have their own limitations. Recently, baculovirus has been developed as a display system using the virus itself or infected insect cells as the display platform. Here, we review the development and use of baculovirus‐infected cells as a platform for display libraries of peptides bound to major histocompatibility complex (MHC) class I (MHCI) or class II (MHCII). We have used fluorescent multimeric soluble T‐cell receptors (TCRs) to screen these libraries and to identify peptide antigen mimotopes. We also present some improvements to this system that allow very large libraries to be constructed and screened. We have used these libraries to examine the role of MHCII‐bound peptides in the presentation of the staphylococcal enterotoxin A (SEA) and to manipulate an MHCI tumor‐associated antigen.