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CXCL8: a potential novel marker in predicting contact sensitisers
Author(s) -
Gibbs Susan,
Toebak MJ,
Pohlmann PR,
Blomberg BME,
Scheper RJ,
Rustemeyer T,
Bruynzeel DP
Publication year - 2004
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.0105-1873.2004.0309dz.x
Subject(s) - ccl17 , cd86 , chemokine , interleukin 8 , allergen , immunology , ccl20 , ccl5 , allergic contact dermatitis , chemistry , chemokine receptor , medicine , allergy , t cell , inflammation , immune system , il 2 receptor
Cultured monocyte‐derived dendritic cells (moDC) have been shown to provide a promising alternative for identifying potential sensitisers in vitro. Allergen induced moDC maturation results in elevated expression of the cell surface markers CD83 and CD86. Unfortunately, this increase in CD83 and CD86 expression is not sensitive enough to identify moderate and weak contact sensitisers. In this study, we investigated whether chemokine production by moDC is increased upon maturation and, ultimately, whether chemokine production can be used as a sensitive method to discriminate allergens from irritants. Methods: MoDC were exposed for 48 hours to allergens (NiSO4, CoCl2, PdCl2, CuSO4, CrCl3, K2Cr2O7, PPDA and DNCB) and irritants (SDS, DMSO, BCl2 and propan‐1‐ol). CD83 and CD86 expression was analysed by flow cytometric determination and chemokine production (CXCL8, CCL5, CCL17 and CCL20) was determined by ELISA. Results: Exposure to strong allergens caused up‐regulation of CD83 and CD86 expression whereas exposure to irritants did not. Remarkably, increased CXCL8 production by moDC was detected after allergen exposure whereas a decrease in CXCL8 production was observed after irritant exposure. CCL20 production was induced only by NiSO4. CCL5 and CCL17 production was increased upon exposure to both allergens and irritants. Conclusion: CXCL8 production by moDC can be used to distinguish allergens from irritants. This chemokine is a potentially novel marker for determining the sensitising capacity of a chemical in vitro. Furthermore, our results suggest an important role for CXCL8 in the sensitization phase of ACD.