Premium
FS04.2
Immunotoxic effects of arylamine dyes
Author(s) -
Skazik Claudia,
Wilbers L,
Breuer S,
Merk HF,
Kunz D,
Blömeke B
Publication year - 2004
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.0105-1873.2004.0309ak.x
Subject(s) - chemistry , polyclonal antibodies , allergic contact dermatitis , cd8 , t cell , cytotoxic t cell , microbiology and biotechnology , immunology , stereochemistry , allergy , biochemistry , in vitro , immune system , antigen , medicine , biology
Allergic reactions to arylamine dyes are well‐known causes for allergic contact dermatitis (ACD). These compounds are found in synthetic and natural materials, hair dyes, sun screen, photographic additives as well as temporary tattoos. Cross‐reactions between different para‐compounds have been frequently observed but the underlying mechanisms are not fully elucidated. We investigated the immunogenic capacities of p‐phenylendiamine (PPD), Bandrowski’s base (BB), 2‐methyl‐1,4‐phenylendiammoniumsulfat (PTD), 4‐aminoazobenzene (AAB), disperse orange (DO), disperse yellow (DY), 4–4‐diaminophenylmethan (DDM), bismarckbrown (BBY) and 2‐mercaptobenzothiazole (MBT) by measuring the proliferative responses of T‐cells on polyclonal and monoclonal level (thymidine incorporation). T cell responses were based on CD4+ and CD8+ T cells. Recently, we characterized PPD/BB T cell clones (TCC) as predominantly CD4+/CD45RO+ cells with a alpha/beta T cell receptor (Sieben et al., 2002). We isolated T cell lines (TCL, n = 16) and T cell clones (TCC, n = 20) from totally 11 PPD‐allergic persons and 5 exposed persons. We established TCLs and TCCs to 4 para‐amino compounds. We found some PPD and/or BB specific TCCs with no cross‐reactivity to the other compounds tested. Some TCCs showed a narrow range of cross‐reactions (BB, BBY, MBT), while others reacted to 6 different para‐amino compounds. We observed similiar patterns of crossreaction for the TCLs. The stimulation indices (SI) varied between 2.2 and 14.0. Our preliminary results on cross‐reactions between para‐amino compounds support recent findings in mice (Wulferink et al., 2002) demonstrating a limited discriminatory capacity of T cells carrying an alpha/beta T cell receptor. Further studies on the mechanisms of cross‐reactivities and the cytokine profiles are now under investigation. This study was supported by the Interdiciplinary Center for Clinical Research and Biomaterials (IZKF, Germany) and the Deutsche Forschungsgemeinschaft (Germany).