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Epitheliotrophic capacity of a growth factor preparation produced from platelet concentrates on corneal epithelial cells: a potential agent for the treatment of ocular surface defects?
Author(s) -
Hartwig Dirk,
Harloff Susanne,
Liu Lei,
Schlenke Peter,
Wedel Thilo,
Geerling Gerd
Publication year - 2004
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.0041-1132.2004.04079.x
Subject(s) - hepatocyte growth factor , growth factor , platelet , wound healing , stimulation , platelet derived growth factor , cell growth , vascular endothelial growth factor , microbiology and biotechnology , immunology , chemistry , andrology , biology , platelet derived growth factor receptor , medicine , cancer research , endocrinology , biochemistry , vegf receptors , receptor
BACKGROUND: Topical application of serum eye drops has been reported to accelerate healing of persistent ocular surface defects. It is supposed that growth factors in serum support the wound healing process. Platelets (PLTs) are rich in growth factors and easily available as PLT concentrates (PCs) from blood banks. Therefore, growth factor preparations from PCs may serve as a new and superior therapeutic agent for such defects. STUDY DESIGN AND METHODS: After thrombin stimulation for growth factor release, the cell‐free supernatant (PLT releasate) of washed PCs (n = 8) was analyzed for epitheliotrophic factors and its wound healing capacity in comparison to serum (n = 8). Human corneal epithelial cells were used as a model to investigate cell growth, migration, and differentiation in response to both blood products. RESULTS: PLT releasate contains more epithelial growth factor, PLT‐derived growth factor, and transforming growth factor‐β, but less hepatocyte growth factor, fibronectin, and vitamins. Cell growth was significantly better in response to PLT releasate. Migration and differentiation were slightly better supported by serum. CONCLUSION: Possibly owing to its high content of growth factors, PLT releasate has a distinct superior effect on cell growth. Stimulation of migration and differentiation was slightly inferior but still acceptable. PLT releasate could therefore be a novel treatment option for ocular surface defects.