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Pfaffia mosaic virus : a new potyvirus found infecting Pfaffia glomerata in Brazil
Author(s) -
Mota L. D. C.,
Della Vecchia M. G. S.,
Gioria R.,
Kitajima E. W.,
Rezende J. A. M.,
Camargo L. E. A.,
Amorim L.
Publication year - 2004
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.0032-0862.2004.01001.x
Subject(s) - biology , potyvirus , chenopodium , myzus persicae , potato virus y , virology , botany , turnip mosaic virus , virus , potyviridae , plant virus , aphid , weed
In Brazil plants of Pfaffia glomerata with mosaic symptoms were found to be infected with a previously undescribed potyvirus, Pfaffia mosaic virus (PfMV). Virus particles were long and flexuous, c. 10 × 700–800 nm, and cylindrical inclusions typical of potyviruses were present in cells of infected tissue. Partial host‐range studies revealed that in addition to P. glomerata , PfMV infected only Chenopodium amaranticolor and Chenopodium quinoa . It was efficiently transmitted by the aphids Aphis gossypii and Myzus persicae . Polyclonal antiserum produced against the PfMV coat protein (CP) reacted with Potato virus Y (PVY), but not with four other potyviruses in PTA‐ELISA. The similarity of the nucleotide sequence of the PfMV coat‐protein gene ( CP ) varied from 7 to 76% when compared with other members of the family Potyviridae . Similarity of the 3′ NTR sequence varied from 4 to 23%. In both cases the highest similarity was with PVY. These data indicate that PfMV is a new species in the genus Potyvirus .