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A SIMPLE AND RAPID TECHNIQUE FOR THE ISOLATION OF DNA FROM MICROALGAE 1
Author(s) -
Fawley Marvin W.,
Fawley Karen P.
Publication year - 2004
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.2004.03-081.x
Subject(s) - biology , dna , dna extraction , isolation (microbiology) , chromatography , algae , cartridge , breakage , polymerase chain reaction , botany , biochemistry , gene , microbiology and biotechnology , materials science , chemistry , metallurgy , composite material
A simple method for the purification of PCR‐ quality DNA from microalgae is presented. This method uses the detergent dodecyltrimethylammonium bromide coupled with cell breakage by agitation in the presence of glass beads and chloroform. A final purification step involves a commercial cartridge system. The procedure requires only about 1–2 mL of algal culture and can be completed in about 20 min. DNA suitable for PCR has been obtained from several algal lineages using this method, including numerous green algae and stramenopiles.