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IDENTIFICATION OF CYLINDROSPERMOPSIN IN APHANIZOMENON OVALISPORUM (CYANOPHYCEAE) ISOLATED FROM LAKE KINNERET, ISRAEL 1
Author(s) -
Banker R.,
Carmeli S.,
Hadas O.,
Teltsch B.,
Porat R.,
Sukenik A.
Publication year - 1997
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1997.00613.x
Subject(s) - cylindrospermopsin , aphanizomenon , cylindrospermopsis raciborskii , biology , fractionation , botany , cyanobacteria , environmental chemistry , chromatography , chemistry , anabaena , bacteria , genetics
Aphanizomenon ovalisporum (Forti) was identified and isolated from Lake Kinneret upon its first appearance as a dominant bloom in late 1994. This cyanobacterial species, not previously known to be toxic, was evaluated by a commonly used mouse bioassay and was demonstrated to induce toxic symptoms that were distinguishable from the typical symptoms of the neurotoxins previously reported in Aphanizomenon flos‐aquae (L.) Ralfs. Mice died 5–24 h after crude extracts were injected intraperitoneally, and the LD 50 value was estimated as 465 mg dry wt biomass · kg −1 mouse. A toxicity‐guided fractionation of the active extract indicated that the potent substance is polar an nature. The structure of the active compound was determined by its mass spectrometry and NMR data. The compound was found to be the sulfate‐guanidinium zwitterion, cylindrospermopsin, previously isolated from the cyanobacterium Cylindrospermopsis raciborskii (Woloszynska) and recently also reported in Umezakia natans (Watanabe). This is the first time that Aphanizomenon ovalisporum has been reported to contain a toxic compound.