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RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS (RAPDs) AS TOOLS FOR GENE MAPPING IN CHLAMYDOMONAS EUGAMETOS (CHLOROPHYTA) 1
Author(s) -
Haring Michel A.,
Schuring Frank,
Urbanus Jos,
Musgrave Alan,
Ende Herman,
Ferris Patrick J.
Publication year - 1996
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1996.01043.x
Subject(s) - biology , locus (genetics) , genetics , restriction fragment length polymorphism , genetic marker , gene , rapd , gene mapping , chlamydomonas , genetic linkage , chromosome , genotype , genetic diversity , population , demography , sociology , mutant
Studying the sexual behavior of the heterothallic green alga Chlamydomonas eugametos Moewus has revealed genetic differences among the few available strains: different O‐ methylated sugar patterns on plasma membrane glycoproteins (oms A/B locus) and light‐(in)dependent sexual agglutinin activation (lsa locus). However, due to the lack of a reliable linkage map, genes controlling these traits are not accessible by map‐based cloning. Here we present a partial linkage map for Chlamydomonas eugametos based on random amplified polymorphic DNA (RAPD) markers and one restriction fragment‐length polymorphism (RFLP) marker. Most of these RAPDs (80%) represent unique DNA sequences, which implies that they can be used as starting points for chromosome walking. RAPDs linked to the lsa locus, the mating type locus (mt) , and the oms A/B locus were identified by bulk segregant analysis.