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A METHOD FOR EXTRACTION OF HIGH MOLECULAR WEIGHT DNA FROM THE MACROALGA PORPHYRA YEZOENSIS (RHODOPHYTA) 1, 2
Author(s) -
Kitade Yukihiro,
Yamazaki Satoru,
Saga Naotsune
Publication year - 1996
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1996.00496.x
Subject(s) - biology , porphyra , dna , rnase p , extraction (chemistry) , restriction enzyme , genomic dna , biochemistry , algae , nuclease , dna extraction , chromatography , botany , rna , polymerase chain reaction , gene , chemistry
We developed a method for extraction of DNA from the red alga Porphyra yezoensis Ueda. The method consists of three preparation steps that include CsCl‐gradient ultracentrifugation, cetyl trimethyl ammonium bromide treatment, and a final RNase step. The amount of DNA extracted from 1.5 g of starting material averaged 17.7 μg. The resulting DNA had a high molecular weight, was 25‐166 kb in length, was digested with five common restriction enzymes, and showed no nuclease activity. It was of sufficient quality for construction of genomic libraries.