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GENERAL ULTRASTRUCTURE AND FLAGELLAR APPARATUS ARCHITECTURE OF WOLOSZYNSKIA LIMNETICA (DINOPHYCEAE)
Author(s) -
Roberts Keith R.,
Hansen Gert,
Taylor F. J. R.
Publication year - 1995
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1995.00948.x
Subject(s) - ultrastructure , biology , flagellum , basal body , transverse plane , anatomy , vesicle , dinophyceae , biophysics , microtubule , electron microscope , physics , optics , microbiology and biotechnology , paleontology , membrane , ecology , genetics , phytoplankton , nutrient , bacteria
The ultrastructure of Woloszynskia limnetica Bursa was examined using serial thin section electron microscopy. Sections of W. limnetica reveal numerous chloroplast profiles without any obvious pyrenoids. The extensive pusular complex consists of a “smooth” part and a part lined with electron‐dense particles. The nucleus is located in the episome. A stigma (= eyespot) consisting of numerous electron‐dense globules is situated beneath the amphiesmal vesicles of the sulcal groove. The longitudinal microtu‐bular root extends between the stigma and the amphiesma vesicles. Subthecal fibers occur in conjunction with the microtubules and the stigma. Both flagellar exit apertures are encircled by a broad striated collar, each giving rise to a fiber that extends along the pusular canal opening. The striated collars are interconnected by the ventral ridge fiber. The basal part of the transverse flagellum has, in addition to the normal paraxonemal rod (= striated strand or fiber), a semicircular structure consisting of fibrils. The flagellar apparatus is complex but possesses components typically found in the Dinophyceae. The longitudinal mi‐crotubular root is broad and is connected to both striated collars. The transverse basal body gives rise to the transverse microtubular root, which in turn is associated with microtubules that extend to the interior of the cell and with the transverse striated root. The transitional region of both basal bodies possesses a distinctive fibrous ring attached to each microtubular triplet by short fibers that collectively appear as spokes of a wheel. Not unexpectedly, the flagellar apparatus of Woloszynskia limnetica is much like that of the related Woloszynskia sp.; however, some dif ferences were discovered. A phylogenetic relationship between Woloszynskia limnetica, W. coronata ( Wolosz.) Thompson, and W. sp. is indicated based on similarities in pusule and stigma structure .