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SPECTRAL CHARACTERIZATION OF NEW CHLOROPHYLL C PIGMENTS ISOLATED FROM EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) BY HIGH‐PERFORMANCE LIQUID CHROMATOGRAPHY 1
Author(s) -
Garrido José L.,
Zapata Manuel,
Muñiz Susana
Publication year - 1995
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/j.0022-3646.1995.00761.x
Subject(s) - emiliania huxleyi , phytol , biology , pigment , chlorophyll , chlorophyll a , chromatography , botany , chlorophyll c , algae , chemistry , phytoplankton , chlorophyll fluorescence , nutrient , organic chemistry , ecology
Pigment extracts from the prymnesiophyte Emiliania huxleyi ( Lohm.) Hay & Mohler (CCMP 370) were analyzed using a new chromatographic method based on the use of polymeric octadecylsilica columns, which split both the chlorophyll c 3 peak and phytol‐substituted chlorophyll c ‐like peak into two fractions. The pigments corresponding to each of these fractions were isolated. The spectra and chromatographic behavior suggest that chlorophyll c 3 from E. huxleyi is a mixture of two different compounds whereas the splitting of the phytol‐substituted c‐like pigment could be the result of the equilibrium of two forms showing the same absorption spectrum. The method allowed, as well, the detection of magnesium 3,8‐divinylpheoporphy‐rin a, monomethyl ester in the prymnesiophytes E. huxleyi ( CCMP 370) and Prymnesium parvum Carter (CCMP 708) and the chrysophyte Pelagococcus subviridis Norris (CCMP 1429 ).